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Optical pacing of Cor.4U® Cardiomyocytes on the Maestro/Lumos™

Most cardioactive drugs affect the spontaneous beat rate of hiPSC-derived cardiomyocytes. Since the repolarization duration of hiPSC-derived cardiomyocytes is dependent on the beat rate, it is necessary to standardize the beat rate. This can be achieved by optical pacing using the Xpress.4U™ LightPace Cor.4U® Kit.

Application

Cardiac safety assessment using optical pacing of Cor.4U® Cardiomyocytes

Certain compounds are known to exhibit frequency-dependent potencies to affect cardiomyocyte properties. Compounds in which a higher beat rate increases their effect are known as use-dependent compounds. Reverse use-dependent compounds however, show an increased efficacy at lower beat rates. Only by controlling the beat rate of the hiPSC-derived cardiomyocytes can the frequency dependence of compounds be analyzed.

This application note describes the assessment of pro-arrhythmic compound effects in optically stimulated Cor.4U® Cardiomyocytes on Axion BioSystems' Maestro and LUMOS™ multiwell light delivery systems. Below an example is shown of reverse use-dependence of dofetilide (a hERG potassium channel Ikr) blocker in optically paced Cor.4U® Cardiomyocytes using the Xpress.4U™ LightPace Cor.4U® Kit.

Reverse use-dependence of dofetilide in Cor.4U Cardiomyocytes

Reverse use-dependent effect of hERG channel blocker dofetilide on the field potential duration of Cor.4U® Cardiomyocytes paced at 1.5-3.0 Hz.

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