x

Pluricyte®
Cardiomyocyte Kit

Pluricyte® Cardiomyocyte Kit is developed and manufactured for use in drug discovery and development. The kit includes human Pluricyte® Cardiomyocytes and Pluricyte® Cardiomyocyte Medium.

 

Product Information


Pre-clinical studies of drug effects with human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes provide novel possibilities for the assessment of efficient and reliable safety and efficacy drug screenings. Pluricyte® Cardiomyocytes are fully functional hiPSC-derived cardiomyocytes obtained without any genetically modification or purification/selection procedures. 
Pluricyte® Cardiomyocytes are cultured in Pluricyte® Cardiomyocyte Medium, a serum free, well-defined culture and maturation medium.

 

Product Data

Product specifications

Pluricyte® Cardiomyocytes are ventricular cardiomyocytes differentiated from human-induced pluripotent stem cells without any genetically modification or purification/selection procedures. The cells show highly-organized, aligned sarcomere structures. 
First contractions of Pluricyte® Cardiomyocytes appear between 24-48 hours post-thawing. It will take 3-4 days before the cells have formed an electrically coupled monolayer. Stable beating monolayers can be observed 7-8 days post-thawing (15-40 BPM). The optimal time window to perform electrophysiology-based assays with Pluricyte® Cardiomyocytes is between 8-12 days after plating the cardiomyocytes. Due to their relatively low beat rate, Pluricyte® Cardiomyocytes can be easily paced for electrophysiological research.

Catalog number

PCK-1.5

Production Technology

Scalable; large lot sizes with minimal batch to batch variation guaranteed by an extensive quality control on identity and functionality

Purity

>70% based on cardiac Troponin T expression. High purity of ventricular cardiomyocytes based on high MLC2v expression and manual patch clamp

Morphology

High degree of structural sarcomere organization

Assay window

Day 8 to 12 post-thaw

Single cell action potential

Low resting membrane potential (~-78 mV), fast upstroke velocity,
robust action potential amplitude

Monolayer field potential

Well-pronounced and robust de- and repolarization peaks,
enabling easy detection of field potential durations and
facilitating MEA assay analysis for pro-arrhythmia drug liability assessment studies.

Recommended Storage

Pluricyte® Cardiomyocyte Medium: -20°C, after opening store at 2-8°C for a maximum of 14 days, no freeze-thaw cycles recommended

Pluricyte® Cardiomyocytes: liquid nitrogen vapor phase