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Xpress.4U LightPace
Cor.4U® Kit

The Xpress.4U LightPace Cor.4U® Kit contains LightPace mRNA, Cor.4U® Cardiomyocytes and Complete Culture Medium, and all proprietary reagents needed for fusion reaction, which have been manufactured under strict QC conditions. The Kit comes equipped with a fully optimized and validated protocol, including a specific pacing protocol, as well as a Quick Guide for the lab.

 

Product Information

Most cardioactive drugs affect the spontaneous beat rate of hiPSC-derived cardiomyocytes. Since the repolarization duration of hiPSC-derived cardiomyocytes is dependent on the beat rate, it is necessary to standardize the beat rate. This can be achieved by optical pacing using the Xpress.4U LightPace Cor.4U® Kit.

Product Data

Product Specifications

 

Catalog number(s)

Nc-S-X-LP

Xpress.4U Technology

The Xpress.4U™ LightPace technology is based on fusion of the mRNA containing vesicles with the cell membrane thus avoiding early lysosomal degradation and induction of stress pathways

Cor.4U®

Cardiomyocytes

To guarantee optimal results, specific Cor.4U® Cardiomyocyte batches have been selected for use with Xpress.4U™ LightPace

Assay Window

Pacing of transfected Cor.4U® Cardiomyocytes can be performed 1-3 days after transfection, with an optimal pacing responsiveness 2 days after transfection

Complete Kit

1 Kit contains enough reagents to transfect 2 Lumos™ OptiClear 48 well plates. Contents: LightPace mRNA; Neutralization buffer; Liposomes; Dilution buffer; Cor.4U® Cardiomyocytes; Cor.4U® Complete Culture Medium

Recommended Storage

Xpress.4U™ LightPace Cor.4U® reagents: -80°C

Cor.4U® Complete Culture Medium: -20°C

Cryopreserved Cor.4U® Cardiomyocytes: liquid nitrogen vapor phase

Validated Application

Validated for use on Maestro and Lumos™ multiwell light delivery systems (Axion BioSystems)

 

Comment

An ultrasonic bath is required

Documentation

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