Get in touch with our experts Ralf and Greg.
Certain compounds are known to exhibit frequency-dependent potencies to affect cardiomyocyte properties. Compounds in which a higher beat rate increases their effect are known as use-dependent compounds. Reverse use-dependent compounds however, show an increased efficacy at lower beat rates. Only by controlling the beat rate of the hiPSC-derived cardiomyocytes can the frequency dependence of compounds be analyzed.
This application note describes the assessment of pro-arrhythmic compound effects in optically stimulated Cor.4U® Cardiomyocytes on Axion BioSystems' Maestro and LUMOS™ multiwell light delivery systems. Below an example is shown of reverse use-dependence of dofetilide (a hERG potassium channel Ikr) blocker in optically paced Cor.4U® Cardiomyocytes using the Xpress.4U™ LightPace Cor.4U® Kit.
Reverse use-dependence of dofetilide in Cor.4U Cardiomyocytes
Reverse use-dependent effect of hERG channel blocker dofetilide on the field potential duration of Cor.4U® Cardiomyocytes paced at 1.5-3.0 Hz.
Download complete application note: