Organ-on-Chip is an interesting technology for mimicking human physiological conditions for drug target discovery, early screening, and drug toxicity testing. Organ-on-a-chip is a multi-channel 3-D microfluidic cell culture chip that simulates the physiological response of complete organs and organ systems. Applying hiPSC-derived (disease) models make these assays even more physiologically relevant.


How it works

Angiogenesis is taken as an example. hiPSC-derived endothelial cells are grown in a microfluidic channel and upon triggering with a gradient of proangiogenic factors, sprouting cells form an intricate capillary network in an adjacent 3-dimensional matrix. The angiogenesis assay platform (48 devices per plate) is compatible with automated systems for cell plating and imaging making it suitable for secondary screening.

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Angiogenesis, the sprouting of new blood vessels from pre-existing vasculature plays a role in normal physiological processes but has also been identified as an important target for treatment of a variety of diseases, including cancer. We have integrated our hiPSC-derived vascular endothelial cells into a state-of-the-art microfluidics system to screen candidate drugs targeting the formation of new blood vessels. Studying the effect of candidate compounds on sprouting can be assessed with this Angiogenesis Assay. Below example shows the concentration-dependent inhibitory effect of the anti-angiogenic compound sunitinib on endothelial cell sprouting.

Representative images of angiogenic sprouts of hiPSC-derived endothelial cells after 48 hours with various concentrations of Sunitinib (top). (Yellow = F-actin, blue = nucleus). Quantitative analysis demonstrate the effect of 50 nM Sunitinib (bottom). (Data obtained in collaboration with Mimetas and LUMC)

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