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Advanced reprogramming services
for customized iPSC lines

You Focus on Advancing Your Research, Let Ncardia Handle Reprogramming

Custom iPSCs as Easy as 1,2,3: 

1) Select donor- or patient-specific material. 
2) Ncardia sources and reprograms it into high-quality induced pluripotent stem cells (iPSCs).
3) We deliver your iPSCs directly to you or further differentiate them into your desired cell type.

 

Get Your Reprogrammed iPSCs in as Little as 12 Weeks

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Expertise, Precision and Reliability in iPSC Generation

Expert Team

100+ cell lines reprogrammed from human, mouse and primate sources

Cutting-Edge Equipment

Automated processes and robust supply chain logistics

Shorter Development Time

Get your iPSC line in just 12 weeks

CASE STUDY: See How Ncardia Delivered 5 Independent Clones From 8 iPSC Lines

Our client, a world-leading pharmaceutical company, required a reliable and consistent disease modeling solution that could serve as a more accessible and reproducible alternative to human tissue.

iPSC Sourcing iPSC Reprogramming Cryopreservation and Delivery

Sourcing, expansion and quality assessment of starting material

iPSCs were derived using human peripheral blood mononuclear cells (PBMCs) obtained from patients.

Before expansion and reprogramming, Ncardia's team assessed:

 

  • Cell counts and viability
  • Mycoplasma
  • STR analysis
  • Genomic stability

 

 

 

 

Reprogramming and Quality Control

Reprogramming was done with non-integrating virus, and first structures appeared after 2 weeks.

Colony picking was done by selecting single-cell derived clones and cells were expanded to ensure enough volume for downstream applications.

 

In-process Quality Control consisted of:

 

  • Post-thaw viability and cell recovery
  • Morphology of colonies
  • Flow cytometry analysis of pluripotency markers
  • Genomic integrity
  • Cell authentication
  • Sterility

5 independent clones from 8 iPSC lines

After quality control, cells were cryopreserved to ensure their long-term viability and differentiation capacity.

 

Five independent clones were delivered to the client and banked at Ncardia for long-term supply to client.

 

What Could Ncardia's Reprogramming Services do for Your Research?

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Let's start a conversation
about your needs

As more and more drug innovators look to capitalize on the unique value only iPSCs can deliver, they look to Ncardia as their qualified collaborator to join them.

Karin, Scientist iPSC technology expert
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