A successful drug efficacy screening and/or validation study requires not only a physiologically relevant cell model, but also validated HTS protocols to test the effect of drug candidates. To achieve this, scientists at Ncardia have developed a controlled approach for large-scale manufacturing of iPSC-derived (disease) cells and automated platforms for cell culturing in a 384-well microplate format, assay readout, and data handling. Assay performance is continuously evaluated using putative quality-controlled criteria such as S/B ratio, Z-prime and %CV to ensure robustness of high throughput compound screenings.
Figure 1: High throughput screening automation robot.
New capabilities and developments are continuously required to pave the way for pharmaceutical companies to expand effective screening or validatation to tens of thousands of molecules in relevant disease model systems. Ncardia’s human induced stem cell-based cellular models in combination with automated phenotypic screening enables reliable efficacy testing of drugs at a high throughput to advance the drug discovery and development process. Our goal is to employ out state-of-the art technologies in our client's research efforts to help deliver better drugs to patients faster.
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Mordwinkin NM, Burridge PW, Wu JC. A review of human pluripotent stem cell-derived cardiomyocytes for high-throughput drug discovery, cardiotoxicity screening, and publication standards. J Cardiovasc Transl Res. Vol. 6, 1, P 22 – 30 (2013)
Picture reference: https://www.ppscreeningcentre.com/wp-content/uploads/2017/10/robot-full-cmyk.jpg