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Resources

Ncardia is committed to delivering working assay solutions, through in-house assay development and customized support, to its clients. We are dedicated to provide you with the support and resources you need. Our scientific support team is knowledgeable in areas including but not limited to electrophysiology, biochemistry and contraction based assays for predictive safety pharmacology and toxicology testing. Do not hesitate to talk to our experts today!

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Key publications

2018

  • Silbernagel N, Walecki M, et al. The VAMP-associated protein VAPB is required for cardiac and neuronal pacemaker channel function. FASEB Journal

  • Hu, N., Wang, T., Wan, H., Zhuang, L., KETTENHOFEN, R., Zhang,X., Zhang, Y.S., Xu, W., Gossmann, M., BOHLEN, H., Hou, X., Wang, P.. Synchronized electromechanical integration recording of cardiomyocytes. Biosensors and Bioelectronics

  • Goineau S, Castagné V. Electrophysiological characteristics and pharmacological sensitivity of two lines of human induced pluripotent stem cell derived cardiomyocytes coming from two different suppliers. J Pharm Tox Meth

  • Baburina I, Varkevisser R, Schramm A, et al. Dehydroevodiamine and hortiamine, alkaloids from the traditional Chinese herbal drug Evodia rutaecarpa, are IKr blockers with proarrhythmic effects in vitro and in vivo. Pharm. Res.

  • De Lorenzo C, Paciello R, Riccio G, et al. Cardiotoxic effects of the novel approved anti-ErbB2 agents and reverse cardioprotective effects of ranolazine. OncoTargets and Therapy

  • Kao I, Xiong Y, Steffen A, et al. Preclinical In Vitro Safety Investigations of Submicron Sized Hemoglobin Based Oxygen Carrier HbMP-700. Art Org

  • Cunha-Oliveira T, Ferreira LL, Coelhoa AR, Deusa CM, Oliveira PJ. Doxorubicin triggers bioenergetic failure and p53 activation in mouse stem cell-derived cardiomyocytes. Tox App Pharm

  • Yamazaki D, Kitaguchi T, Ishimura M, et al. Proarrhythmia risk prediction using human induced pluripotent stem cell-derived cardiomyocytes. J Pharm Sci

  • Tertoolen LGJ, Braam SR, van Meer BJ, Passier R, Mummery CL. Interpretation of field potentials measured on a multi electrode array in pharmacological toxicity screening on primary and human pluripotent stem cell-derived cardiomyocytes. Biochemical and Biophysical Research Communications

  • Ncardia's CNS.4U® utilized within a 10-tissue micro-physiological system: Edington CD, Chen WLK, Geishecker E, et al. Interconnected Microphysiological Systems for Quantitative Biology and Pharmacology Studies. Scientific Reports

  • Ncardia Conference Report. Mulder P, de Korte T, Dragicevic E, Kraushaar U, Printemps R, Vlaming MLH, Braam SR, and Valentin JP. Predicting cardiac safety using human induced pluripotent stem cell-derived cardiomyocytes combined with multi-electrode array (MEA) technology: A conference report. J Pharmacol Toxicol Methods

  • Kopljar I, Hermans AN, Teisman A, Gallacher DJ, and Lu HR. Impact of calcium-sensitive dyes on the beating properties and pharmacological responses of human iPS-derived cardiomyocytes using the calcium transient assay. J Pharmacol Toxicol Methods

  • Nawroth C, Scudder LL, Halvorson RT, Tresback J, Ferrier JP, Sheehy SP, Cho A, Kannan S, Sunyovszki I, and Goss JA. Automated fabrication of photopatterned gelatin hydrogels for organ-on-chips applications. Biofabrication

  • Goversen B, Becker N, Stoelzle-Feix S, Obergrussberger A, Vos MA, van Veen T, Fertig N, and de Boer TP. A Hybrid Model for Safety Pharmacology on an Automated Patch Clamp Platform: Using Dynamic Clamp to Join iPSC-Derived Cardiomyocytes and Simulations of Ik1 Ion Channels in Real-Time. Frontiers in Physiology

2017

  • Ligneau X, Shah RR, Berrebi-Bertrand I, et al. Nonclinical cardiovascular safety of pitolisant: comparing International Conference on Harmonization S7B and Comprehensive in vitro Pro-arrhythmia Assay initiative studies. Br J Pharmacol.

  • Lei CL, Wang K, Clerx M, Johnstone RH, Hortigon-Vinagre MP, Zamora V, Allan A, Smith GL, Gavaghan DJ, Mirams GR and Polonchuk L. Tailoring Mathematical Models to Stem-Cell Derived Cardiomyocyte Lines Can Improve Predictions of Drug-Induced Changes to Their Electrophysiology. Frontiers in Physiology

  • Kopljar I, De Bondt A, Vinken P, Teisman A, Damiano B, Goeminne N, Van den Wyngaert I, Gallacher D, Lu, HR. Combining Video Microscopy Motion Analysis and Detection of Cardiac Injury Biomarkers to Evaluate Chronic Cardiotoxicity in hiPS Cardiomyocytes. Journal of Pharmacological and Toxicological Methods. 88. 239-240. 10.1016/j.vascn.2017.09.233

  • Bodi I, Grünert SC, Becker N, Stoelzle-Feix S, Spiekerkoetter U, Zehender M, Bugger H, Bode C, Odening KE. Mechanisms of acquired long QT syndrome in patients with propionic academia. PMID: 26854997

  • Kujala VJ, Pasqualini FS, Goss JA, Nawrotha JC, Parker KK. Laminar ventricular myocardium on a microelectrode array-based chip. Journal of Materials Chemistry B.
  • Rehnelt S, Malan D, Juhasz K, Wolters B, Doerr L, Beckler M, Kettenhofen R, Bohlen H, Bruegmann T, Sasse P. Frequency-Dependent Multi-Well Cardiotoxicity Screening Enabled by Optogenetic Stimulation. PMID: 29211031
  • Gélinas R, El Khoury N, Chaix MA, Beauchamp C, Alikashani A, Ethier N, Boucher G, Villeneuve L, Robb L, Latour F, Mondesert B, Rivard L, Goyette P, Talajic M, Fiset C, Rioux JD. Characterization of a Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Model for the Study of Variant Pathogenicity: Validation of a KCNJ2 Mutation. PMID: 29021306
  • Lapp H, Bruegmann T, Malan D, Friedrichs S, Kilgus C, Heidsieck A, Sasse P. Frequency-dependent drug screening using optogenetic stimulation of human iPSC-derived cardiomyocytes. PMCID: PMC5575076
  • Polonchuk L, Chabria M, Badi L, Hoflack J-C, Figtree G, Davies MJ, Gentile C. Cardiac spheroids as promising in vitro models to study the human heart microenvironment. PMCID: PMC5539326
  • Batista L, Bastogne T, Delaunois A, Valentin V-P, Atienzar F. A novel statistical signal processing method to estimate effects of compounds on contractility of cardiomyocytes using impedance assays. HAL Id : hal-01621227
  • Rana P, Luerman G, Hess D, Rubitski E, Adkins K, Somps C. Utilization of iPSC-derived human neurons for high-throughput drug-induced peripheral neuropathy screening. J Pharmacol Toxicol Methods. 2017 Dec. PMID: 28843493
  • Pugsley MK, de Korte T, Authier S, Huang H, Accardi MV, Curtis MJ. Safety pharmacology methods and models in an evolving regulatory environment. J Pharmacol Toxicol Methods. 2017 Sep. PMID: 28461240
  • L.G.J. Tertoolen, S.R. Braam, B.J. van Meer, R. Passier, C.L. Mummery. Interpretation of field potentials measured on a multi electrode array in pharmacological toxicity screening on primary and human pluripotent stem cell-derived cardiomyocytes. Biochem Biophys Res Commun. 2017 Jan 30. PMID: 28153730
  • Devarasetty M, Forsythe S, Shupe T D, Soker S, Bishop C E, Atala A and Skardal A. Optical Tracking and Digital Quantification of Beating Behavior in Bioengineered Human Cardiac Organoids. Biosensors (Basel). 2017 Jun 23. PMID: 28644395
  • Koci B, Luerman G, Duenbostell A, Kettenhofen R, Bohlen H, Coyle L, Knight B, Ku W, Volberg W, Woska JR, Brown MP. An impedance-based approach using human iPSC-derived cardiomyocytes significantly improves in vitro prediction of in vivo cardiotox liabilities. Toxicol Appl Pharmacol. 2017 Aug 15. PMID: 28546047
  • Franz D, Olsen H L, Klink O, Gimsa J. Automated and manual patch clamp data of human induced pluripotent stem cell-deriveddopaminergic neurons. Sci Data. 2017 Apr 25. PMID: 28440808
  • Zhang N, Milleret V, Thompson-Steckel G, Huang NP, Vörös J, Simona BR, Ehrbar M. Soft Hydrogels Featuring In-Depth Surface Density Gradients for the Simple Establishment of 3D Tissue Models for Screening Applications. SLAS Discov. 2017 Jun. PMID: 28277889

2016

  • Zhao L., Zhang B. Doxorubicin induces cardiotoxicity through upregulation of death receptors mediated apoptosisin cardiomyocytes. Sci Rep. 2017 Mar 16. PMID: 28300219
  • Johan U. Lind, Travis A. Busbee, Alexander D. Valentine, Francesco S. Pasqualini, Hongyan Yuan, Moran Yadid, Sung-Jin Park, Arda Kotikian, Alexander P. Nesmith, Patrick H. Campbell, Joost J. Vlassak, Jennifer A. Lewis and Kevin K. Parker. Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing. Nat Mater. 2017 Mar. PMID: 27775708
  • Blinova K, Stohlman J, Vicente J, Chan D, Johannesen L, Hortigon-Vinagre MP, Zamora V, Smith G, Crumb WJ, Pang L, Lyn-Cook B, Ross J, Brock M, Chvatal S, Millard D, Galeotti L, Stockbridge N, Strauss DG. Comprehensive Translational Assessment of Human-Induced Pluripotent Stem Cell Derived Cardiomyocytes for Evaluating Drug-Induced Arrhythmias. Toxicol Sci. 2017 Jan. PMID: 27701120
  • Hortigon-Vinagre MP, Zamora V, Burton FL, Green J, Gintant GA, Smith GL. The Use of Ratiometric Fluorescence Measurements of the Voltage Sensitive Dye Di-4-ANEPPS to Examine Action Potential Characteristics and Drug Effects on Human Induced Pluripotent StemCell-Derived Cardiomyocytes. Toxicol Sci. 2016 Dec. PMID: 27621282
  • Hartmann T, Overhagen S, Ouwens DM, Raschke S, Wohlfart P, Tennagels N, Wronkowitz N, Eckel J. Effect of the long-acting insulin analogues glargine and degludec on cardiomyocyte cell signalling and function. Cardiovasc Diabetol. 2016 Jul 15. PMID: 27422524
  • Hertz LD, Owzar K, Lessans S, Wing C, Jiang C, Kelly WK, Patel JN, Halabi S, Furukawa Y, Wheeler HE, Sibley A, Lassiter C, Weisman LS, Watson D, Krens SD, Mulkey F, Renn CN, Small EJ, Febbo PG, Shterev I, Kroetz D, Friedman PN, Mahoney JF, Carducci MA, Kelley MJ, Nakamura Y, Kubo M, Dorsey SG, Dolan ME, Morris MJ, Ratain MJ and McLeod HL. Pharmacogenetic Discovery in CALGB (Alliance) 90401 and Mechanistic Validation of a VAC14Polymorphism that Increases Risk of Docetaxel-Induced Neuropathy. Clin Cancer Res. 2016 Oct 1. PMID: 27143689
  • Monnerat G, Alarcón ML, Vasconcellos LR, Hochman-Mendez C, Brasil G, Bassani RA, Casis O, Malan D, Travassos LH, Sepúlveda M, Burgos JI, Vila-Petroff M, Dutra FF, Bozza M, Paiva CN, Carvalho AB, Bonomo A, Fleischmann BK, Campos de Carvalho AC and Medei E. Macrophage-dependent IL-1β production induces cardiac arrhythmias in diabetic mice. Nat Commun. 2016 Nov 24. PMID: 27882934
  • Kopljar I, Gallacher DJ, De Bondt A, Cougnaud L, Vlaminckx E, Van den Wyngaert I, Lu HR. Functional and Transcriptional Characterization of Histone Deacetylase Inhibitor-Mediated Cardiac Adverse Effects in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Stem Cells Transl Med. 2016 May. PMID: 27034410
  • Goßmann M, Frotscher R, Linder P, Neumann S, Bayer R, Epple M, Staat M, Artmann AT, Artmann GM. Mechano-Pharmacological Characterization of Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells. Cell Physiol Biochem. 2016. PMID: 26983082
  • Mathieu S, El Khoury N, Rivard K, Gelinas R, Goyette P, Paradis P, Nemer M, Fiset C. Reduction in Na+ current by angiotensin II is mediated by PKCα in mouse and human-induced pluripotent stem cell–derived cardiomyocytes. Heart Rhythm Journal 2016 Feb. PMID: 26921763
  • Bodi I, Grünert S, Becker N, Stölzle-Feix S, Spiekerkoetter U, Zehender M, Bugger H, Bode C, Odening KE. Mechanisms of acquired long QT syndrome in patients with propionic academia. Heart Rhythm Journal 2016 Feb. PMID: 26854997
  • Yvonne Aratyn-Schaus, Francesco S. Pasqualini, Hongyan Yuan, Megan L. McCain, George J.C. Ye, Sean P. Sheehy, Patrick H. Campbell, and Kevin Kit Parker. Coupling primary and stem cell-derived cardiomyocytes in an in vitro model of cardiac celltherapy. Cell Biol. 2016 Feb. PMID: 26858266
  • Ralf Frotscher, Danita Muanghong, Gözde Dursun, Matthias Goßmann, Ayşegül Temiz-Artmann, Manfred Staat. Sample-specific adaption of an improved electro-mechanical model of in vitro cardiac tissue. J Biomechanics 2016 Feb. PMID: 26972766

Newsletters

2018

2017

Posters

2018

Posters SoT 2018
(1) Simultaneous Measurement of Contractility, Electrophysiology, and Troponin I Secretion to Determine Cardiotoxicity in hiPSC-Derived Cardiomyocytes

(2) Use of Cor.4U® hiPS-Derived Cardiomyocytes to Detect Drug-Induced Mitochondrial Toxicity - Impact of Cell Culture Medium

(3) Optimization of a Human Stem Cell-Derived Neuron/Astrocyte Co-Culture System for Seizure Liability Assessment Using Microelectrode Arrays

Posters SLAS 2018
(1) Voltage and calcium sensitive dye recording from hiPSC-derived cardiomyocytes using the Hamamatsu FDSS plate reader (for (96 well) cardiac safety assessment/assay) to assess drug-induced cardiac liabilities

In collaboration with Servier

(2) High throughput assessment of cardiotoxicity in hiPSC-derived cardiomyocytes

2017

Posters SOT meeting 2017
(1) Assessment of electrophysiology, contractility and viability effects of three UCB compounds on human induced pluripotent stem cell-derived (hiPSC) cardiomyocytes using the Real Time Cell Analyzer (RTCA) CardioECR platform In collaboration with UCB Biopharma

(2) Towards prediction of acute and chronic drug effects using multi-parameter profiling of hiPSC-derived cardiomyocytes

(3) Investigating Drug Induced Cardiomyocyte Dysfunction through Combined Analysis of Beating, Metabolic Flux and Cellular Oxygenation

Posters ISSCR international symposium 2017
(1) Application of human-induced pluripotent stem cell-derived cardiomyocytes in high-throughput screening assays for drug safety and efficacy testing

(2) Human iPSC-derived cardiomyocytes: A translational model to predict drug-induced cardiac arrhythmias and long-term toxicity

Posters SLAS 2017
(1) High-throughput assessment of drug effects on calcium transients in human induced pluripotent stem cell (hiPSC) derived cardiomyocytes

(2) High-Throughput Automated Patch Clamp Complemented by Optical Stimulation

(3) Investigating Cardiomyocyte Dysfunction through Combined Analysis of Beating, Metabolic Flux and Cellular Oxygenation

Poster EMBL 2017
Mechanistic Investigation of Cardiomyocyte Dysfunction through Combined Analysis of Beating, Metabolic Flux and Cellular Oxygenation

Poster ELRIG 2017
High-Content Assay for Morphological Characterization of Neuronal Development in 3D Matrix Using Human iPSC-Derived Neuronal Cultures

Poster SCDD 2017
Human iPSC-derived cardiomyocytes: A translational model to predict drug-induced cardiac arrhythmias and long-term toxicity

Poster J. Pharm. Tox. Meth. 85:88-89 May 2017

The late sodium current participates in repolarization of Cor.4U® human iPSC-derived cardiomyocytes

Posters SPS 2017

(1) Towards Prediction of Acute and Chronic Drug Effects using Multi-parameter Profiling of hiPSC-derived Cardiomyocytes

(2) Multiparametric Assessment of Cardiotoxicity of Chemotherapeutic Drugs in Human Induced Pluripotent Stem Cell (hiPSC)-Derived Cardiomyocytes

(3) Studying Inotropic Compound Effects in Relatively Mature Human iPSC-derived Cardiomyocytes using 2D and 3D Models

(4) Application of Human-Induced Pluripotent Stem Cell Derived Cardiomyocytes in High-Throughput Screening Assays for Drug Safety and Efficacy Testing

(5) Neurotransmitter Screening via Dynamic Calcium Flux and MEA-based Seizure Liability Assessment in Human iPS Neurons

(6) Unblinded: Responses to CiPA 28 Compounds in Cor.4U Cardiomyocytes

(7) Opening the Molecular Toolbox: Co-transfection Of KCNJ2 and Channelrhodopsin2 mRNAs for Precise Control of iPS-Cardiomyocyte Pacing and Functionality

(8) Characterization of Electrical and Optical Paced hiPSC-derived Cardiomyocytes in vitro Using Multiwell Microelectrode Array Technology (co-presented with Axion BioSystems)

(9) Pluricyte® Cardiomyocytes as a Predictive Tool for Preclinical Safety Assessment, a Comparison with Isolated Guinea pig Ventricular Cardiomyocytes (co-presented with PhysioStim)

(10) Comprehensive Profiling of Axiogenesis Ventricular vCor.4U iPSC-derived Cardiomyocytes – From Electrophysiology to Phenotypic Assays (co-presented with Metrion Biosciences)

 

2016

Posters SPS meeting 2016

(1) Assessment of in vitro compound-induced pro-arrhythmia in Pluricyte® Cardiomyocytes

(2) Multiparametric assessment of the effects of TKIs on the (electro)physiology of Pluricyte® Cardiomyocytes

(3) Characterization of electrical and mechanical functions of Pluricyte® Cardiomyocytes using CellOPTIQ® In collaboration with Clyde Biosciences

(4) Assessment of compound effects on electrophysiology of Pluricyte® Cardiomyocytes using patch clamp and MEA – In collaboration with PhysioStim

(5) Assessment of electrophysiology, contractility and viability effects of three UCB compounds in Pluricyte® Cardiomyocytes – In collaboration with UCB Biopharma

 

Poster FDSS User Meeting 2016
Assessment of drug effects on electrophysiology and contractility of Pluricyte® Cardiomyocytes

Poster ISSCR Meeting 2016
Pluriomics’ Pluricyte® Cardiomyocyte Differentiation Kit

Poster MEA Meeting 2016
In vitro pro-arrhyhtmia detection in Pluricyte® Cardiomyocytes using multiple MEA assays

Poster SPS meeting 2015
MEA based electrophysiological characterization of Pluricyte® Cardiomyocytes

Poster US Cardio Symposium 2015
Using Pluricyte® Cardiomyocytes in the xCELLigence CardioECR platform