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Ncyte® Heart in A Box™



Ncyte® Heart in a Box™ is an innovative 3D cardiac microtissue model derived from human-induced pluripotent stem cells (iPSCs). It integrates three critical cell types—ventricular cardiomyocytes, endothelial cells, and cardiac fibroblasts—into a single, high-purity product that mirrors the complexity of the human heart. 

This system provides a more accurate platform for studying heart development, disease modeling, and therapeutic testing.

cardiac

Immunofluorescence staining for α-SMA (red), cTnT (yellow), and PECAM (green) showing the presence of key cell types. DAPI (blue) stains nuclei.

Benefits
  • Comprehensive cardiac model
  • High purity and functionality
  • Supports co-culture applications to study cell interactions within the cardiac microenvironment
  • Enables advanced toxicity and drug screening studies

Do you want to buy Ncyte® Heart in a Box™?

Do you need a different cell volume or a custom model?

Product Specifications

Identity markers

vCardiomyocytes: ≥90%cTnT+, Endothelial Cells: ≥90% CD31+/CD144+, Cardiac Fibroblasts: ≥95% vimentin+/≥85% TCF21, Thawing according to user guide

Format

Cryopreserved cells

Size (viable cells/vial)

vCardiomyocytes: ≥4M viable cells, Endothelial Cells: ≥1M viable cells, Cardiac Fibroblasts: ≥ 0.4M cells viable cells, Thawing cells according user guide

Quality Control

Cell count, Viability, Identity (FACS, ICC), Mycoplasma testing

Donor

Female

Reprogramming method

Ectopic expression of reprogramming factors using episomal plasmids

Shipping conditions

Dry shipper, -180C to -135C

Storage conditions

Vapor phase of liquid nitrogen

Technical Data

Learn more about Ncyte® Heart in a Box™ by downloading the fact sheet

Cardiotoxicity Testing and Drug Screening Heart Disease Modeling Angiogenesis and Vascular Remodeling Fibrosis Research and ECM Remodeling

Cardiotoxicity Testing and Drug Screening

Ncyte® Heart in a Box™ offers a comprehensive platform for assessing the cardiotoxic effects of potential drug candidates. By incorporating cardiomyocytes, endothelial cells, and cardiac fibroblasts, this system allows researchers to evaluate drug effects on cardiac function, viability, and toxicity across various heart cell types. High-throughput screening and large-scale compound testing can predict cardiotoxicity risks before clinical trials, reducing the reliance on animal models.

B: Response of 3D Cardiac Microtissues to tool compounds
Disease modeling with Ncardia’s cardiac microtissues: CPVT.

Heart Disease Modeling

This system is ideal for modelling various
cardiovascular diseases, including ischemic
heart disease, heart failure, hypertrophy, and
fibrosis. Researchers can explore disease
mechanisms in a human-relevant 3D envi-
ronment, gaining a deeper understanding of
cellular interactions and disease progression.
Ncyte® Heart in a Box™ is particularly useful
for studying how cardiomyocytes, endothelial
cells, and fibroblasts interact during heart
disease development.

Response to Drugs in the Heart in a Box Model

Angiogenesis and Vascular Remodeling

Ncyte® Endothelial Cells form functional
vascular networks, providing an excellent
model for studying angiogenesis and vascular
remodelling, especially in ischemic heart
diseases. This application is valuable for
testing therapies aimed at promoting blood
vessel growth and improving circulation,
such as in post-myocardial infarction or
heart failure.

Ac-LDL uptake assay showing Ncyte® Endothelial Cells’ ability to internalize acetylated low-density lipoprotein

Fibrosis Research and ECM Remodeling

Cardiac fibrosis, a hallmark of many heart
diseases, is driven by the activation of car-
diac fibroblasts. Ncyte® Cardiac Fibroblasts
offer a unique opportunity to study ECM re-
modelling, collagen deposition, and fibroblast
activation in response to injury. This system
is critical for developing antifibrotic therapies
to reduce scar tissue formation and restore
heart function.

Immunofluorescence staining for vimentin (green) and connexin 43 (CX43, red). Vimentin is a cytoskeletal protein present in fibroblasts, while CX43 is a gap junction protein involved in cell-to-cell communication. DAPI (blue) stains the cell nuclei. Scale bar: 50 μm
Immunofluorescence staining for collagen type I (COL1A1, red) and CX43 (green). COL1A1 is a major component of the extracellular matrix. DAPI (blue) stains the cell nuclei.
Product Information and Applications

 

Certificates of analysis are available upon request via support@ncardia.com

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Questions about our cell models?

Our work centers on a simple yet powerful premise: 
When we combine deep iPSC knowledge, broad assay capabilities and a demonstrated ability to integrate the biology of human diseases into preclinical research, we can help drug developers make critical decisions earlier and with more confidence.