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Ncyte® aCardiomyocytes

Human iPSC-derived atrial-like cardiomyocytes

Atrial cardiomyocytes play a crucial role in cardiac function, not just by contracting to pump blood, but also through their hormone production, electrical properties, and Ca2+ signaling.

Ncyte® aCardiomyocytes are a powerful tool to improve in vitro testing of new treatments for atrial arrhythmias. The high purity and consistency of this model can help you increase reproducibility of your results. The functionality of the cells enables it’s application in high-throughput assays for large-scale screening with minimal variability.

cardiac
Immunostaining with cardiac marker cTnT (green) and specific atrial marker COUP-TFII (red). Nuclei stained with DAPI (blue).
Benefits
  • Avoid lot-to-lot variability
  • Functional and physiologically relevant
  • Suitable for electrophysiological studies

Do you want to buy Ncyte® aCardiomyocytes?

Do you need a different cell volume or a custom model?

Product Specifications

Identity markers

≥ 70% cTnT+ cells and ≥ 70% COUP-TFII+ cells from cTnT+ population at day 3 of culture according to user guide

Size

≥ 2M viable cells at day 3 of culture according to user guide

Quality Control

Cell count, viability, identity, human pathogen screening, mycoplasma

Format

Cryopreserved cells

Donor

Female

Reprogramming method

Ectopic expression of reprogramming factors using episomal plasmids

Shipping conditions

Dry shipper, -180°C to -135°C

Storage conditions

Vapour phase of liquid nitrogen

Optional Supply of

Ncyte® Cardiomyocytes Medium

Technical data

Identity Suitable for Electrophysiology Suitable for drug screening

 

 

Ncyte® aCardiomyocytes are characterized by flow cytometry to ensure a high purity, assessing double expression of cardiac Troponin T (cTnT) and the specific COUP transcription factor II (COUP-TFII), as shown in the figure.

Flow cytometry analysis of one representative batch of Ncyte® aCardiomyocytes 3 days after thawing
Immunofluorescence staining of Ncyte® aCardiomyocytes 3 days after thawing, showing double expression of cTnT (green) and the specific atrial marker COUP-TFII (red). DAPI (blue).

Ncyte® aCardiomyocytes show atrial-like action potentials and high electrical activity as measured by MEA analysis. They show a stable beat rate with very low inter- and intra-well variation. This model is, therefore, physiologically relevant for the phenotypic study of atrial arrhythmias.

Our expert scientists can develop and execute tailor assays to support your drug discovery research in the cardiac field.

MEA analysis demonstrates that Ncyte® aCardiomyocytes present robust re- and depolarization peak amplitudes, critical for fast and accurate data analysis.
Analysis of over 50 beats demonstrate high stability and consistent beating patterns.

Ncyte® aCardiomyocytes are suitable for electrophysiological research and drug discovery. In this example, they show relevant pharmacological responses to known cardioactive compounds with acute and chronic treatments (culturing according to project needs).

Our scientists are also able to create and refine custom assays to evaluate the efficacy and safety of new therapeutic candidates using this model.

Chronic drug-induced cardiotoxicity: concentration- and time-dependent decrease of depolarization peak amplitudes and number of active electrodes after treatment with Doxorubicin
Product Information and Applications

 

Certificates of analysis are available upon request via support@ncardia.com

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Questions about our cell models?

Our work centers on a simple yet powerful premise: 
When we combine deep iPSC knowledge, broad assay capabilities and a demonstrated ability to integrate the biology of human diseases into preclinical research, we can help drug developers make critical decisions earlier and with more confidence.