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Cortical Neurons

Human iPSC-derived cortical neurons

Cortical neurons play a vital role in many neurodegenerative diseases, including Alzheimer’s disease. Human iPSC-derived cortical neurons can be co-cultured with astrocytes and microglia to model neurodegenerative diseases. Our team can manufacture cortical neurons at scale from the iPSC line that best fits your project to support your drug discovery campaigns.

neural

Cortical neurons

Cellular Identity Physiologically Relevant Highly suitable for drug discovery

Ncyte Cortical Neurons are post mitotic, glutamatergic, cortical layer-committed neurons that display branching phenotypes, early in culture. We define the cells fit-for-purpose based on the identification of several biomarkers. Ncyte Cortical neurons contain ≥85% ßIII Tubulin and ≥50% CTIP2-positive cells after 14 days of postthaw culture. They express the cortical progenitor marker FOXG1 and cortical layer V and VI marker TBR1, together with dendrite marker MAP2. Further identification based on other biomarkers can be perfomed upon request.

Ncyte Cortical Neurons express neuronal marker βIII Tubulin (red) and cortical neuron marker TRB1 (green) 7 days after thawing.

Ncyte Cortical Neurons express neuronal marker βIII Tubulin (red) and cortical neuron marker Ctip2 (green) 7 days after thawing.

Ncyte Cortical Neurons express neuronal marker βIII Tubulin (red) and cortical neuron marker MAP2 (green) 7 days after thawing.

Ncyte Cortical Neurons exhibit spontaneous activity on multi-electrode arrays (MEA). Firing and bursting properties are presented as early as day 4 post-thaw, which evolve into more complex structured patterns at later time points. Because of this fast maturation process, our team of neuroscientists can start performing your experiments relatively soon, enabling shorter lead times with reduced well-to-well variation.

Human iPSC-derived cortical neurons display spontaneous activity on MEA. Burst-like spontaneous activity on MEA obtained for hiPSC-derived cortical neurons two weeks after thawing (B).

Co-culturing of Ncyte Cortical Neurons with Ncyte Astrocytes provides a relevant model to study neuroinflammation responses. In addition, our scientists can use this cell line to model Parkinson's disease and support in vitro testing throughout drug discovery campaigns.

Multi-electrode array (MEA) raster plots of cortical neurons at day 14 post-thawing in absence (A) and presence of astrocytes (B). The overall neuronal activity increases in the co-culture with astrocytes.